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The presence of PEP and Mg^sup 2+^ did not change substantially the secondary structure of the protein, as indicated by FTIR measurements signature loan rate .
In experiments with the effectors (5 mM Mg^sup 2+^ and 2 signature loan rate .
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The activation energy E^sub a^ was determined from the slope of these plots with the accuracy of 5-8 kcal/mol, which includes the fitting errors and the deviations among different data sets signature loan rate .
There was, however, strong evidence (2-tailed t-test, P<0 signature loan rate .
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