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MATERIALS AND METHODS Materials Sodium dodecyl sulfate (SDS) was purchased from Sigma (St
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At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range
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The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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Finally, they were cleaned by ethanol and dried properly to remove any contamination
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The RT^sup n^^sub 1^ values for the CaM:SEF2-1mp complex are superimposed onto the S^sup 2^ contours in Fig
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Supernatant solutions were decanted, and carbonate precipitates were washed free of soluble salts by repeated decantations with deionized water
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Reactivity (%)EggSpecies (positive egg no
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