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. Lower concentration rates of protein to trypsin were used (100:1, 200:1, and 300:1), but in all these experiments the amount of nondigested EI^sup sc^ in the absence of effectors was insignificant after 1 min of reaction (data not shown)
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. Consequently, the k^sub ex^ values calculated in this way (Eqs
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. The RT^sup n^^sub 1^ value is always 1 in the absence of internal motion or when the internal motion is faster than ~200 ps
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