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. Finally, the solution was dialyzed against TBS (Tris buffer (pH 7
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. There may be several factors ascribed to the lower yield of the protein, including the oligomeric nature of the protein and its glycosylation
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. This scale invariance, referred to as dilatation symmetry (39) is a hallmark of chains in the EV limit and rollouts the preservation of the hierarchical nature of contact patterns irrespective of sequence or chain length
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. After 2 h, only very small aggregates (400 h), the protofibrils have increased in length to 100-200 nm, and the spherical clusters have decreased in size to 20-30 nm in diameter (Fig
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. The fluorescence lifetime of ANS was found to be the longest and therefore the most reliable in trying to resolve rotational correlation times of TTR oligomers and larger assemblies
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. 9-meter Anglo-Australian Telescope in Coonabarabran confirmed that hunch, he says
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. As expected, the T^sub g^ of our EVA copolymers lies between the T^sub g^ values of the monomers (polyethylene -125°C, polyvinylacetate 31°C [3])
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