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This suggests that the exposure of one SH group in the presence of 1 M GdnHCl is due to an ionic strength effect
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A total of 27 hydrogen-bonding interactions and 53 hydrophobic interactions were found to stabilize the structure, which suggests that hydrophobic interactions between the protein and the glycans are responsible for the exceptionally high stability of the glycoprotein
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Native-like mean structure in the unfolded ensemble of small proteins
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We carried out measurements at different protein concentrations either by CD and fluorescence in the concentration range 4-8 µM at pH 7
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Although glycosylation does not affect the structure, protein dynamic fluctuations decrease globally, decreasing amide hydrogen exchange rates by up to sixfold throughout the entire protein
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This property is only related to the chemical nature of the flux and additives and is independent of the solid contents of the flux
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After amplification and discrimination, signals were directed to an ALV/5000E Multiple Tau Digital Correlator and time-intensity correlation functions recorded, typically for 600 s duration
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The fluorescent dye, 4,6'-diamidino-2-phenylindole (DAPI) was obtained from Wako Pure Chemical Industries (Osaka, Japan)
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Structural mapping of an aggregation nucleation site in a molten globule intermediate
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Similar multiphase kinetics of the Gdm-induced unfolding was observed in apoA-1:DMPC disks (Reijngoud and Phillips, 1982) and in apoC-1 disks (unpublished data)
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Inhibition of virus-induced cell fusion by apolipoprotein A-I and its amphipathic peptide analogs
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Partitioning the configurational space in this way, and by providing an attractive "gutter", relying on local forces alone, to connect one diffusive subspace to the next, creates the advantageous funnel landscape when the high-dimensional structure is projected into a one- or two-dimensional space
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When key stabilizing interactions succeed in reducing the dimensionality of the search space more drastically, much higher values can result (from differentiation of the higher-dimensional analogs of Eq
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Im7 folding mechanism: misfolding on a path to the native state
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While astronomers had conjectured that light from surrounding stars might release hydrogen, they hadn't expected it to have enough energy to ionize it, Malin says
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For a curing time of 3 minutes, the EVA 9% toner flowed well at all three temperatures and formed a film over the fabric surface, but did not coat the fibers
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1 million Pharmacare spent on NSAIDs for seniors in the 12 months prior to Type 2 RP
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