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. The spectra were measured after the samples had been equilibrated for 2 min at given temperatures controlled with a circulating water bath
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. With this caveat in mind, we assert that both the scaling exponent v and the intercept R^sub o^ calculated in the EV limit show statistically significant agreement with estimates from SAXS data (11)
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. The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift
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. Indeed, engineered HuBChE with a reduced degree of glycosylation displays catalytic properties similar to those of the wild-type enzyme (62)
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. For all other cases, the move is rejected
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