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Asymmetry in the shapes of folded and denatured states of proteins
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In the EI protein from Salmonella typhimurinm
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Zaccai*||* Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale, Grenoble, France; [dagger] Centre de Recherches du Service de Santé des Armées, Unité d'Enzymologie, La Tronche, France; [double dagger] Institut de Pharmacologie et Biologie Structurale, Toulouse, France; § Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland; ¶ Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel; and || Institut Laue-Langevin, Grenoble, FranceSubmitted February 9, 2005, and accepted for publication June 30, 2005
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Indeed, the activity of compounds for inducing DNA phase separation is generally determined by their DNA binding potential
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Protein compactness measured by fluorescence resonance energy transfer
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.Mass spectrometryThe BLG preparation was examined by liquid chromatography-mass spectroscopy using reverse phase chromatography with on-line electrospray-ionization mass spectrometry (MicroMass, Cary, NC) as described previously (14)
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. Kinetic evidence of an on-pathway intermediate in the folding of lysozyme
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