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. The pretransitional structural changes involved the partial unfolding of the solvent-exposed β-sheet structures and the transformation of about half of the Class I Trp fluorophores to Class II
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. Reversibility of the thermal transition was examined by reheating the samples after cooling from the first scan
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. And finally, thermal denaturalions were carried out to test for changes in protein stability upon binding to the effectors
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. For example, at 20 µm ANS where binding sites are fully saturated in solution and the intensity has reached its maximum value, the corresponding intensity in ice is merely 3%-5% of the value in the liquid water
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. Although the protein globule may carry a zero net charge, its surface charge distribution is heterogeneous and the net charge reflects the balance between positive and negative charges
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. 0 the virial coefficient remained negative as also found by Schaink and Smit (40) using membrane osmometry (mixed BLG A and B) at pH 5
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. Linear viscoelasticity of colloidal hard-sphere suspensions near the glass-transition
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. 0) at 4°C for 96 h
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. Irregularly shaped particles that could not be divided into spherical particles were produced for cases D and E (similar cooling rates)
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. Similarly, Raczynski and Ruprecht (1974) showed 8-21% underrepresentation of individual mammalian prey in pellets of three species of European owls
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. A 1989 Gallup poll revealed that of the adult population who reported they had gambled during the previous 12-month period, 70% reported having gambled three or more times per week, with as many as 6% considered probable "compulsive gamblers" (Netemeyer, et al
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. The value of R^sub o^ obtained from fits to SAXS data for denatured proteins suggests that denatured proteins show rodlike behavior over very short length scales (11)
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. Here, a^sub o^ is a normalization constant, which ensures that ∫^sup ∞^^sub 0^ 4πx^sup 2^P(x)dx = 1
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. The concept of a random coil: residual structure in peptides and denatured proteins
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. Therefore, the maximum of the fluorescence emission spectrum (λ^sub max^) is sensitive for the polarity of the microenvironment of the intrinsic fluorophore
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