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Design of a hyperstable protein by rational consideration of unfolded state interactions union federal savings and loan .
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The tetrameric enzyme has nine glycosylation sites per monomer, and carbohydrates account for ~25% of its dry weight union federal savings and loan .
The occurrence of such states depends upon the range and strength of the attractive and repulsive forces acting between the particles union federal savings and loan .
2-4, the osmotic pressure determined using the osmotic stress technique is plotted as the dimensionless compressibility factor, Π/ρk^sub B^T, as a function of protein mass fraction for pH 8 union federal savings and loan .
Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0 union federal savings and loan .
In practice, the large difference in anisotropy decay between monomeric forms lead to a broad baseline with the contribution of the smaller monomeric forms contributing as a decay curve superimposed onto a baseline union federal savings and loan .
Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes union federal savings and loan .
The observation of 30% helical structure in 5-20 µg/ml apoA-2 in the absence of significant self-association, and the unfolding of this structure upon cooling (Fig union federal savings and loan .
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5, in which we assume that the transition state energy depends on denaturant concentration in the same way as the gutter energy, for the sake of illustration union federal savings and loan .
Isopar G, H, and L contain hydrocarbons with 10-11, 11-12, and 11-13 carbon atoms, respectively, and were used as received union federal savings and loan .
The inability of two- and three-way helical junctions to populate stable quasi-docked conformations appears to be a key determinant for the reduced stability and incomplete tertiary folding observed in the corresponding ribozymes union federal savings and loan .
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