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5) with a final concentration of 1 mg/ml
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Assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclodextrins
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5 M denaturant concentration also exibits a loss in the value of the mean residue ellipticity, suggesting that there is a loss in the secondary structure of the protein (Fig
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Hence, the first transition is related to the dissociation of the tetrameric unit to constituent monomers, whereas the second one represents opening up of the individual subunits
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A twofold advantage underlies our choice of sort-core repulsions
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In this work, we set n = 14
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How random is a highly denatured protein
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Since then, Xenopus oocytes and other large single cells like Aplysia californica neurons (300 µm in diameter) have been used in numerous quantitative water MRI studies that mapped the apparent diffusion coefficients or spin-spin and spin-lattice relaxation times of intracellular water (9-13)
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75 ml g^sup -1^ (35) were assumed
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The viscous behavior of globular protein solutions will therefore be strongly influenced by the characteristics of this hydration layer
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They showed that DNA compaction by both monocations is preferred at higher temperature; however, the slopes of these dependencies were different as a result of different thermodynamic parameters of DNA compaction
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Independently from producing evidence for the step-resolved character of the process, intermediate and morphologically heterogeneous forms were trapped and characterized, which yields direct evidence for the multipathway character of the amyloidogenesis of insulin
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McLeishDepartment of Physics and Astronomy and Astbury Centre for Molecular Biology, University of Leeds, Leeds, United KingdomSubmitted November 4, 2003, and accepted for publication September 7, 2004
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In addition to the changes directly related to the intermolecular contact, CD4 binding also induces conformational changes away from the binding interface that are crucial for the subsequent events of viral entry into the host cell
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The V3 loop of gp120 shows a monotonous increase of the average configurational entropy difference (circles in Fig
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The objectives of the research [14] reported in this paper are to produce toners using binders with high flexibility (low T^sub g^ and high elongation) and to evaluate the properties of fabrics printed with these toners
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