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. Hence, the limit of a perfect solvent is also referred to as the EV limit (39)
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. This helix-loop-helix motif folded against the ban-el, covering a hydrophobic patch (which includes Tip^sup 461^): the motif was linked to the barrel by two salt bridges, namely, Arg^sup 537^-Asp^sup 467^ and Arg^sup 546^-Asp^sup 459^ (Fig
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. 1 are all in nonhelical regions except residues 72, 73, and 75, which are pail of the less well-defined C-terminal part of helix IV
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. Akke and co-workers (38) have also found larger ΔH^sup #^ values for residues that are part of regular secondary structure and smaller values for loop residues, in their study on the C-terminal domain of CaM
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. The amount of aggregated material, or the composition of the coexisting phases, was measured by centrifuging the samples for 5 min at 4000 g, removal of the upper phase, and determination of protein content of the aggregates or the concentration in each coexisting phase using spectrophotometry
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. Densely packedwithout sexual activityoogonial nests andand yellowish (except fordeveloping oocytes, withvery small fish) duringlarger oocytes containingmonths with reproductive small clear vesicles
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. Protein concentration was determined by the Lowry method (Lowry et al
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. The sample with the protein and the effectors was incubated for a minimum of 2 h at room temperature
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. Once thermograms were normalized to protein concentration, a chemical baseline calculated from the progress of the unfolding transition was subtracted
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. We have previously found that activated PLCβ-Gβγ complex can be rapidly deactivated by Gα(GDP) subunits without dissociation, which led to the suggestion that Gα(GDP) binds to PLCβ-Gβγ and perturbs the activating interaction without significantly affecting the PLCβ-Gβγ binding energy
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