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Paul, Minnesota, booed and others walked out when the senior speaker, Ben Kessler, said it is selfish for women to use the pill for contraception
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The purity of stock GR preparations was routinely verified by SDS-PAGE, followed by Coomassie-Blue staining
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Apart from its crucial functions in intracellular amino acid metabolism, ACYI also has substantial commercial importance for the optical resolution of N-acylated DL-amino acids
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The precipitate was removed by centrifugation at 8000 rpm for 30 min
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We find that modeled denatured proteins can be best described as follows
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Digestion was slopped by the addition of 17 µl of SDS-PAGE loading buffer, and samples were immediately run on a gel
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No pH corrections were done for the isotope effects
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Structure and stability of an early folding intermediate of Eschericliia coli Trp aporepressor measured by far-UV stopped-flow circular dichroism and 8-anilino-1-naphthalene sulfonate binding
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The newly frozen samples were then allowed to anneal for 12 h at -5°C, followed by a final equilibration of 2 h at the temperature selected for fluorescence measurements (-5 and -13°C)
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Counteracting effects of thiocyanate and sucrose on chymotrypsinogen secondary structure and aggregation during freezing, drying, and rehydration
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The effect is due to the limitation of the instrumental resolution regarding the measurement of hydration water large-scale motions (40)
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42 s^sup -1^ is obtained, which is very close to the average experimental R^sub 1^
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For example, milling is a surface operation, and it requires a minimum amount of lubricity
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Conjugated linoleic acid (CLA) is another component found only in ruminant products (like cheese)
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The V4 loop (residues 398-409), for which electron density is missing in all crystal structures, and the V3 loop, which has been proposed to undergo significant rearrangements upon CD4 binding, were modeled onto the gp120 core, together with the six core mutations of the LAI strain (98
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They are prescribed in certain cases of delayed puberty or muscle wasting
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Alderson cited Wal-Mart as a leader in helping seniors and their families prepare for Part D
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The phenomenon of stress as perceived by Project 2000 student nurses: a case study
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"Gamers called it Magic crack
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Sure, Finkel finds himself
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David Schwartz, director of the Center for Gaming Research at the University of Nevada--Las Vegas, points out in his 2003 book Suburban Xanadu that Las Vegas succeeded not only because of its reputation as Sin City, an exotic, remote enclave where people could go and behave in ways they wouldn't in the presence of nosy neighbors, but also, and perhaps mostly, because it replicated the home environments of visitors
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Now, like a high-stakes blackjack player doubling down, the president is letting the world know that he is ready to do it all over again in Iran
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But many Bush officials have an innate distrust, even loathing, of China, especially in the office of Vice President Cheney, who in 2001 drew several of his top aides from the staff of a strongly anti-China congressional committee pursuing allegations that Beijing had stolen state secrets during the Clinton administration
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. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein
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. In the final structure, the interface domain is hidden in a hydrophobic, packed core surrounded by the other domains, as demonstrated by crystallographic studies of human GR (Schulz et al
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.8), 150 mM sodium chloride, 15% glycerol, 1 mM Ca^sup 2+^/Mn^sup 2+^, and 200 mM lactose)
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. When the binding reaches saturation, nonbound molecules dominate the diminazene signal in the nucleus
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. In summary, chemical denaturation of discoidal lipoproteins is a complex multiphase transition whose kinetics is distinctly different from that of the thermal denaturation; similar to thermal denaturation, chemical denaturation involves protein unfolding/dissociation (Reijngoud and Phillips, 1982) and lipoprotein fusion (Fig
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.A challenge faced by all these models is that the most successful search for inherent features of tertiary structure that correlate with folding rates has found that the topological measure of "contact order" is far more closely related than, for example, molecular weight itself in the case of "two-state" folders (Plaxco et al
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. In the hypergutter picture, the nine-dimensional search space of these critical regions separately is reduced to three sequential three-dimensional searches by the long lifetimes of the native regions when the remainder of the protein is disordered
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