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. 5, we show backbone traces of 10 EV limit conformations each for four different protein sequences
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. It is interesting that no difference in thermal fluctuations between HuBChE and DmAChE is observed, despite their different catalytic turnover rates
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. 3, we tested internal motion models with either one (M1; τ^sub 1^ and S^sup 2^ fitting parameters) or two contributions (M2; τ^sub if^, S^sup 2^^sub f^, τ^sub is^ and S^sup 2^^sub s^ fitting parameters) to the internal motion
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. An isotyping stick in the kit was incubated with the above solution at room temperature for 15 minutes
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. In fact, by localizing the undocked ensemble close to the native state, the quasi-docked state should reduce the entropie penalty for docking
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., 1988) and facilitation of protein lysosomal degradation (Ptitsyn, 1992)
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. Hence, these scans were not employed tor the calculation of the thermodynamic parameters for the unfolding of proteins
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. In addition, all thermal transitions in NDSB solutions exhibit also the missing reversibility as observed for pure buffer solutions
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. The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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. In addition, using the simple general equation, which covers for both fast and slow exchange, derived by Ishima and Torchia (34), we also show that the exchange rate can be determined from a combination of CPMG measured R^sub ex^ and change in R^sub ex^, (ΔR^sub ex^, vide infra) without prior assumption on the exchange timescale
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.RESULTSCaM R^sub 1^, and R^sub 2^ relaxation rates were measured at 600 and 400 MHz ^sup 1^H frequency and the ^sup 15^N-{^sup 1^H} NOE data at 600 MHz
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. Aggregation of acid unfolded TTR (23°C, 14 µM monomer concentration) was induced by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM
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. Such protofilaments merge and intertwist, yielding thin fibrils, which are capable of further association and twining, producing mature amyloid
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