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Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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The biological relevance of the partially folded species at acidic pH is unknown, and we do not know whether the residual secondary structure detected by CD and FTIR (Table 2) was native-like
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Among a large variety of different methods, such as absorption-, circular dichroism spectroscopy, calorimetry, NMR, and light scattering techniques, in particular fluorescence spectroscopy is a powerful technique to monitor unfolding transitions and to characterize properties of the unfolded state (see for example (3,4))
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. Protein thermal aggregation involves distinct regions: sequential events in the heat-induced unfolding and aggregation of hemoglobin
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. Since conformational fluctuations are uncorrelated in a chain devoid of interactions, it is impossible to find large cavities (a > 5 [Angstrom]) within the interior of a freely rotating chain
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.INTRODUCTIONSignal transduction through heterotrimeric G-proteins is initiated by the binding of an extracellular ligand to its corresponding G-protein coupled receptor (for background, see Alberts et al
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.Amyloidogenic Self-Assembly of Insulin Aggregates Probed by High Resolution Atomic Force MicroscopyJansen, RalfABSTRACTAs the application of high-resolution atomic force microscopy (AFM) has led us recently to the discovery of a unique pressure-induced circular amyloid, we used the same approach to examine morphological events accompanying insulin aggregation under ambient conditions
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. Likewise, the Mg-binding parameters (K^sup Mg^^sub D^ and n) obtained from the previous trFRET data are also in reasonable agreement with the corresponding values reported here
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