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the high mannose GlcNAc^sub 2^Man^sub 9^ glycan chain was attached with Asn-75 (Nδ2) of the protein (Protein Data Bank code 2SBA) using MOE (Molecular Operating Environment) software, and energy was minimized using a force field of AMBER99
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Given a new set of Cartesian coordinates from step 4, we calculate the energy for the new conformation
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Adaptive ImmunityThe strongest evidence linking efferocytosis with the regulation of adaptive immunity is the association of human and murine autoimmune diseases with the impaired removal of apoptotic cells
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Due to a tight sealing of the quartz cuvettes temperatures of protein solutions up to ~120°C were applicable without evaporation and boiling
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To enable characterization of different conformational states of TTR, we employed four different molecular probes: ANS, 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) (Fig
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Denaturation kinetics of apoA-2:DMPC disksTo correlate the heat-induced lipoprotein fusion with the protein unfolding and to probe the kinetics of these transitions, we recorded CD and light-scattering data at 222 nm upon sample heating and cooling at a rate from 1
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, 2002); however, correlation of the CD and 90° light-scattering data in Fig
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As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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We attempted to use a process similar to the one described by Blunt to produce polypropylene toner, but were unsuccessful
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It also recorded the bet size, the total number of points sat on (total sat on), the number of points when the last card was drawn (total drawn on), and the number of play decisions that violated the Basic strategy (i
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