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Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig va housing loan .
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We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane va housing loan .
1, A and B; curve 1, Fig va housing loan .
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The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig va housing loan .
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