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. Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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. The protein was eventually cluted in 0
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. AIP Series in Polymers and Complex Materials
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. No pH corrections were done for the isotope effects
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. 1 shows the change in ANS fluorescence induced by the four azurin species upon freezing of the sample at -13°C (200 µM ANS, 2
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. All frogs used were healthy, and all experimental procedures involving animals were performed in accordance with the institutional ethical guidelines
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. Both the analytical expressions for tumbling times of cylinder symmetric objects as well as bead model hydrodynamics incorporated in the DASHA software (24) were used (see Supplementary Materials for more details)
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. We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane
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. 1, A and B; curve 1, Fig
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. Rough machining of pockets and cavities, as well as threading and tapping operations, are all done early in the process, and then the finishing operations are run
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. The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig
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. The pellets cultured in DMEM/ ITS+/TGF-[beta]1 were larger and contained significantly greater amounts of DNA and glycosaminoglycans
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. However, the low homology among the amino acid sequences of D- and L-aminoacylases available suggests that they may have different fold and catalytic mechanisms (2)
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. Generation of conformational ensembles We have adapted conventional Markov-chain Metropolis Monte Carlo simulation strategies (60,61) to generate equilibrium ensembles for each of the protein sequences in the EV limit
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. This scale invariance, referred to as dilatation symmetry (39) is a hallmark of chains in the EV limit and rollouts the preservation of the hierarchical nature of contact patterns irrespective of sequence or chain length
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. There are two obvious differences between our approaches
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. Address reprint requests to Rohit V
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. Further, the model was evaluated with PROCHECK (37)
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. Although a quantitative relation between aggregation and reduction of the unfolding induced red shifts cannot be given, our data indicate that protein aggregation contributes significantly to smaller magnitudes of red shifts as observed during thermal unfolding
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. There are four known families of PLCβ enzymes and all are activated by Gα^sub q^-subunits (for review, see Rebecchi and Pentyala, 2000; Rhee, 2001)
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