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This roundtable offers viewpoints and advice about ways to seize opportunities to improve the revenue cycle
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. One of the best-documented cases of pleiotropy is the gene associated with sickle-cell disease
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. The solvent-accessibility of tryptophan and tyrosine residues was examined by iodide and acrylamide quenching at several pHs
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. These azurins maintain an intact native fold (x-ray structure) but are ~6 kcal/mole less stable relative to the wild-type (WT) (18-22)
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. Transmission electron microscopyFive microliter aliquots from a TTR misfolding reaction were withdrawn at different time points and were placed on a carbon coated copper grid for 2 min
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. Addition of 7 M GdnHCl completely abolished the CD signal at 222 nm, indicating extensive or complete unfolding of GR
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. As a representative for the first class, BLA shows a λ^sub max^-value for the native state of 333 nm, which increases up to 339 nm upon thermal unfolding
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. A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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.The limitations of the study need to be considered before generalizing the results obtained
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