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. Estefanía Hurtado-Gómez,* Gregorio Fernández-Ballester,* Harald Nothaft,[dagger] Javier Gómez,* Fritz Titgemeyer,[dagger] and José Luis Neira*[double dagger] * Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Alicante, Spain; [dagger] Lehrstuhl für Mikrobiologies, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany; and [double dagger] Biocomputation and Complex Systems Physics Institute, Zaragoza, Spain Submitted October 31, 2005, and accepted for publication March 7, 2006
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. As already mentioned above, the unfolding transition of proteins is not only characterized by the disappearance of the well-defined three-dimensional structure but can also cause distinct changes in the compactness of the unfolded structures or may induce pronounced protein aggregation
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. By assuming equilibrium conditions and an ANS activity in ice equal to the concentration in solution multiplied by a freeze concentration factor of 120 (12), one derives ANS dissociation constants in the range of 6-8 mM for WT, 2-4 mM for C3A/C26A, 2
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. This work was supported by the Research-Infra Construction Program and the National Research Laboratory Program (grants to C
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. The addition of a small amount of Bi (1 wt
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. In the absence of DNA, the basic sequences loose their well-defined secondary structure (14)
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. The fluorescence emission peak has here shifted to 482 nm, which was not noted in the case of increased viscosity
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. This is because the rate-determining diffusive searches will in general be completed only when a topological, as well as a spatial, constraint in the final native state is satisfied for the first time
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. , Amityville, NY, ASTM D1388-64)
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