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To better understand the step-wise changes of the different Trp fluorophores, the spectrum recorded at each temperature was fitted using the discrete states model of Trp residues in proteins (30,32,34)
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Also, LDH, which is notoriously unstable to freezing (14-16), was found to exhibit the largest binding capacity among the proteins examined in this study
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John Wiley & Sons, Chichester
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The fluorescence from ANS and Bis-ANS fluorophores show strict environmental dependence and are essentially nonfluorescent in water but are highly fluorescent in hydrophobic environments
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ANS bound to aggregated TTR showed that the minor component increased its lifetime to 4
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Redetermination of the pressure dependence of the lipid bilayer phase transition
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Studies of the structure of insulin fibrils by Fourier transform infrared (FTIR) spectroscopy and electron microscopy
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Age at 50% maturity was calculated by using our estimate of length at 50% maturity and a VBGF rearranged to the form[t
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. A first-principles reassessment of the source of this empirical correlation is mandated
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. The thermal stability of EI^sup SC^, in the absence of PEP and Mg^sup 2+^, was maximal at pH 7
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. 1 C), with a maximum shifted to lower pH (at pH 6) than that found in fluorescent experiments (Fig
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.Finally, because proteolytic activity, oxidant stress, and TNF-[alpha] all suppress efferocytosis, and are thought to be significant contributors to chronic lung inflammation, we would expect that any therapy directed toward these imbalances may have potential beneficial effects on both efferocytosis and clinical outcomes
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. 6, whereas Table 1 collects the data for residues with significant exchange broadening (see table legend for more details)
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. The interaction is clearly different from the well-known categories of specific binding by lock-and-key or induced-fit binding, in which the complex in the bound state becomes essentially locked in one conformation
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. As the associated shear stress was [much less than]50 kPa, we ascribe this behavior to being equivalent to the η^sub 0^ of hard sphere colloidal suspensions
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. 6 and Table 1) shows:i
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. Protein association to membranes is stabilized by a combination of electrostatics as well as polar and hydrocarbon interactions
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. Interestingly, a closer scrutiny of the insulin aggregates obtained after 1-min incubation at 60°C indicates that clusters of swollen insulin particles tend to align within the immediate vicinity of the early fibers (Fig
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. We showed earlier that the pressureinduced circular amyloid of insulin consists of several loops of a thin and untwisting form, possibly a single protofilament (Jansen et al
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. 3A), small spots were evident along the lower jaw and the ventral part of the head
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. The parameters, such as polymer concentration and cooling rates for phase separation of polyethylene, were quite different from those used in our study to produce IPP and EVA toners
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. J Laryngol Otol 1995;109:1097-1100
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.126 -2,895 23,228OpiatesAverage monthly effect 492 <
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. Page count has been kept about the same by reducing type size
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., Pharmacal Sciences Pharmacy Care Systems Ph
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