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in financial engineering from Virginia Tech, in addition to a B
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The thermal unfolding of ACYI might be a three-state process involving an aggregation-prone intermediate appearing at ~68°C
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The transition curves of the amide I' hands were obtained according to a method described previously (28)
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This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations
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002 µg/µl for trypsin, that is, the final concentration rate between both proteins was 500:1) in a total volume of 17 µl in 100 mM of Tris buffer at pH 7 (27)
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(16) Adenosine triphosphate-binding cassette proteins including ABC-A1, ABC-A7 (A
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Irreversible thermal denaturation of Torpedo californica acetylcholinesterase
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Structural and dynamical features contributing to thermostability in alpha-amylases
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To understand the absence of diminazene signal in both cytoplasmic compartments, macromolecule binding has to be considered once more
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8 and between 5 and 200 ps, respectively
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Circular dichroism spectroscopy and light scatteringCircular dichroism (CD) and light-scattering data were recorded using AVIV-215 spectrometer (AVIV Biomedical, Lakewood, NJ) with fluorescence accessory and Pelletier temperature control
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The CD melting curves at 222 nm, Θ^sub 222^(T), recorded during sample heating and cooling from 1 to 80°C, show that the helical structure reaches maximum at 25°C and completely unfolds upon heating as well as cooling from 25°C (Fig
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4, A and C) is due to a leveling off of the curve for the free form, which suggests that convergence is near for the free molecule
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