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. In addition, the peak corresponding to tryptophan emission displayed a significant red-shift to [lambda]^sub max^ ~ 334-339 nm
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. and for the sake of demonstration the data were fitted to a three-slate unfolding model as described in the analysis section to have a quantitative idea of the thermodynamie parameters accompanying the unfolding of the recombinant nonglycosylated protein
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. The importance of native-state topology for folding is underscored by analysis of the average denatured-state topology
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. Instrumental aspects and data analysisNeutron scattering experiments were carried out on the backscattering spectrometer IN16 (energy resolution ΔE = 0
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. Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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. Frontiers in high pressure biochemistry and biophysics (preface)
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. Comparison with apoA-1 and apoC-1To assess the relative stability of the DMPC complexes with various human apolipoproteins, we used DSC, CD, and light scattering to monitor heat denaturation of similar-size DMPC disks with apoA-1, apoA-2, or apoC-1 under otherwise identical conditions
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. DISCUSSION AND CONCLUSIONSWe have discussed a conceptual approach to the protein landscape problem that attempts to remain faithful to the high dimensionality of the system
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