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. 5 to 4 µM, and only KI was used as the quencher
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. The interaction neither belongs to the category of lock-and-key nor to that of induced-fit
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. Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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. Experimentally these states have been principally observed in colloidal systems although it has been shown that there are strong analogies between the behavior of colloidal and concentrated globular protein solutions (2)
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. 6 kDa and a protein specific volume of 0
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. The aggregation reaction of monomeric acid unfolded TTR (14 µM) was initiated by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM (23°C)
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. Free energy determination of polypeptide conformations generated by molecular dynamics
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. These data are overlaid on the shift plots in Fig
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. --Chemistry Health Policy Pharmaceutics Pharmacology Toxicology Pharmacognosy BiochemistryTemple University School of Pharmacy M
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. This indicates that subtle conformational changes are induced by low concentrations of GdnHCl (The existence of partially folded intermediate states in the unfolding of GR is indicated by a comparison of fluorescence spectral shift, anisotropy, iodide quenching, and CD data as a function of GdnHCl concentration
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