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. This indicates that subtle conformational changes are induced by low concentrations of GdnHCl (The existence of partially folded intermediate states in the unfolding of GR is indicated by a comparison of fluorescence spectral shift, anisotropy, iodide quenching, and CD data as a function of GdnHCl concentration
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. For example, milk-fat-globule-factor (MFG)-E8 and growth-arrest-specific-factor 6 (Gas6) are soluble bridging molecules that directly link PS on the apoptotic cell surface with their corresponding phagocyte receptors; [alpha]v[[beta]
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. A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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.Osmotic stress techniqueThe dependence of the osmotic pressure of BLG solutions on protein concentration, pH, and added NaCl concentration was determined using an osmotic stress technique
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. Furthermore, these data correlate nicely to the crosslinking data of Fig
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. Additionally, little change in membrane polarity and hydration of POPC/PI(4,5)P^sub 2^ (67:33) membranes are detected in this same pressure range (Rebecchi et al
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. The onset of this transition at various scan rates is observed at T^sub R^ = 32-34°C, similar to T^sub R^ interred from the CD data
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